It was the fourth time in five years that Corowa beekeepers experienced mass deaths in their hives, at the same time each year.
Now the NSW Environment Protection Authority (EPA) is investigating potential pesticide misuse after the samples tested positive to the pesticide, fipronil.
The EPA is trying to determine the source of the chemical and is appealing to the public for any information.
Fipronil is a chemical commonly used in agriculture in spraying crops, in pest control and in household insecticides to contain pests like fleas and ticks.
Executive director of operations, Jason Gordon, said the EPA was exploring all avenues for potential causes of the deaths.
“Bees have a large flight diameter from their hives, which makes them difficult to track and we have been working with beekeepers to try to determine potential flight paths,” Mr Gordon said.
“Bees play a crucial role in local pollination, which helps keep ecosystems healthy and food production going and we want to do everything we can to protect them.
“We are now seeking assistance from the public to try and locate the source.”
Pesticide misuse, whether intentional or accidental can be a serious offence, and individuals could face fines and potentially stronger consequences.
Accidental pesticide misuse can occur, such as keeping and using out of date chemicals, so The EPA says it's important to always read labels carefully and follow directions to ensure chemicals like fipronil are used safely and correctly.
Safely and securely storing pesticides, along with properly cleaning and disposing of containers, is essential.
The EPA recommends the use of the BeeConnected app to assist pesticide users in preventing impacts to foraging bees.
Anyone with information about the alleged pesticide misuse should contact the Environment Line on 131 555 or email info@epa.nsw.gov.au.